Hom twgsilicayog siv nyob rau hauv chromatography?
Chromatography yog ib qho txheej txheem tseem ceeb uas siv rau hauv kev tshuaj ntsuam chemistry kom cais thiab txheeb xyuas ntau yam tshuaj lom neeg hauv kev sib tov. Silica gel yog ib qho ntawm cov khoom siv feem ntau siv hauv chromatography vim nws qhov chaw siab thiab polarity. Feem ntau, muaj ob hom silica siv hauv chromatography, uas yog, theem ib txwm thiab thim rov qab theem silica.
Ib txwm theem silica yog ib qho polar nyob ruaj ruaj theem uas yog siv los cais cov tsis-polar analytes. Hom silica no muaj lub ntsej muag hydrophilic uas nyiam cuam tshuam nrog cov kuab tshuaj polar thiab cov tebchaw, uas ua rau kev sib cais ntawm cov tsis muaj polar compounds. Ib txwm theem silica feem ntau yog siv hauv cov kua chromatography (LC) thiab nyias txheej chromatography (TLC) daim ntawv thov.
Ntawm qhov tod tes, thim rov qab theem silica yog qhov tsis yog-polar nyob ruaj khov theem uas yog siv los cais cov polar analytes. Hom silica no muaj qhov chaw hydrophobic uas nyiam cuam tshuam nrog cov kuab tshuaj tsis muaj polar thiab cov tebchaw, uas ua rau kev sib cais ntawm cov polar compounds. Reversed theem silica feem ntau yog siv nyob rau hauv high-performance kua chromatography (HPLC) daim ntaub ntawv.
Ntxiv rau cov theem ib txwm thiab thim rov qab theem silica, kuj tseem muaj lwm yam kev hloov pauv ntawm silica siv hauv chromatography xws li bonded-theem silica thiab ntxeem tau silica hais. Bonded-theem silica yog ib qho silica nto uas tau hloov kho nrog ib pawg tshuaj tshwj xeeb los muab cov kev sib tham tshwj xeeb rau kev sib cais. Porous silica particles muaj qhov tsis sib xws nrog ntau qhov pores uas tuaj yeem ua rau thaj chaw saum npoo av muaj rau kev sib cuam tshuam.
Hauv kev xaus, silica yog siv nyob rau hauv chromatography vim nws qhov chaw siab thiab polarity, thiab muaj ntau hom silica uas siv nyob ntawm seb hom analytes raug cais. Silica yog ib qho khoom siv tseem ceeb siv hauv chromatography uas tso cai rau kev txheeb xyuas thiab ua kom huv ntawm ntau yam tshuaj lom neeg hauv kev sib tov.